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Digital PCR Playbook: Applications and Challenges Across Research and Clinical Labs
Alex Zevin, PhD
Director, Genomics Shared Resource
Fred Hutchinson Cancer Center
Panelist
Alex Zevin, PhD
Alex Zevin, PhD, began serving as the director of the Genomics Shared Resource at Fred Hutch in December 2022. Before that, he was a research scientist at ArcherDX where he developed NGS in vitro diagnostic devices including several clinical trial assays and an approved companion diagnostic. He also previously worked at InBios International and developed a rapid test for detection of anthrax.
Zevin has a bachelor’s degree in biochemistry from Colorado State University and a PhD in molecular biology from Arizona State University where he developed methods to characterize bacterial communities in engineered systems and conducted postdoctoral research at the University of Washington studying host-microbe interactions in non-human primate models.
- Time:
Digital PCR has emerged as a powerful approach for precise nucleic acid quantification, but it is constrained by limited dynamic range and the difficulty of multiplexing. Newer platforms, like Countable Labs’ single-molecule counting PCR, address both by offering precise quantification across a broad range of target abundances while simplifying multiplexing through single-molecule isolation and fluorescent imaging across millions of spatially fixed compartments.
In this GEN webinar, Alex Zevin, PhD, director of Fred Hutchinson Cancer Center’s Genomics Shared Resource, draws on hands-on experience with managing a suite of nucleic acid quantification technologies, including standard qPCR, digital droplet PCR, and Countable PCR, to share practical guidance for labs considering or expanding their PCR quantification capabilities. Key insights from the webinar include:
- How single-molecule counting differs from conventional digital PCR—and the sensitivity, precision, and multiplexing advantages it enables
- Real-world applications suited to single-molecule counting PCR, including validating NGS results, replacing or supplementing existing assays, and generating clinically actionable data
- Common challenges for converting qPCR and dPCR assays to single-molecule counting PCR, and how to overcome them
A live Q&A session will follow the presentations offering you a chance to pose questions to our expert panelist.
Produced with support from:
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